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[Apoptosis induced by interstitial irradiation with 32P glass microspheres combination with hyperthermia in mouse
1Department of Oral Maxillofacial Surgery, College of Stomatology, West China University of Medical Sciences.
Objective:
The aim of this study was to observe the changes of tumor cell apoptosis after interstitial irradiation combination with the hyperthermia treatment.
Methods:
Twenty mice with solid tumor S180 were divided into four groups, including a control group which was given normal saline, and three experimental groups which were respectively applied with a hyperthermia treatment (bath water), interstitial irradiation with 32P glass microspheres, and the combined treatment of hyperthermia and interstitial irradiation. The terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) method was used to determine the final apoptotic cells.
Results:
The mean values of apoptotic indexes of these four groups were respectively 0.39, 0.53, 0.59 and 0.91. The apoptotic indexes of these experimental groups were significantly higher than that of the control group (P < 0.05). The apoptotic index of the combined treatment group was significantly higher than those of the hyperthermia group and the interstitial irradiation group (P < 0.05).
Conclusion:
Both the interstitial irradiated with 32P glass microspheres and hyperthermia (bath water) can induce the apoptosis of mouse solid tumor S180. It seems that there is a synergistic induction of apoptosis between interstitial irradiation and hyperthermia.