[The devitalization of tumor suppressor genes in oral squamous cell carcinoma]

To study the expression of anti-oncogenes nm23, P16, P53 of squamous cell carcinoma within one sample.

A total of 27 cases of oral squamous cell carcinoma came from our the First Clinical College, China Medical University from 1997 to 1998, and none of them had the history of radioactivity therapy or chemical therapy. In them, 8 cases were in the early period, and 19 cases were in the late period. 15 cases were male and 12 cases were female. The samples were fixed in formaldehyde solution, embedded with paraffin, sliced in 5 microns, and analysed by ABC immunohistochemistry staining. The first antibodies were anti-P16, anti-nm23, anti-P53 respectively. The criteria of judgment were the following: For nm23, the case that cytoplasm or nucleus were buffy particles or ball was positive. For P16, the case that nucleus were brown was positive, while the case with the light brown cytoplasm and unstained nucleus was negative. The stained nucleus of interstitial cells were treated as the control. For p53, the positive protein product was assembled in the carcinoma cell nucleus, so the nucleus with buffy or brown particles was positive. According to the criteria, 5 high power sights were selected from each case, more than one hundred cells were counted. The case that the positive cells was more than 30 percentage was high expression, and less than 30 percentage was low expression. Two doctors completed the procedure respectively, and only the same results could be qualified.

In the 27 cases, the devitalization of nm23 and p16 was showed in low expression, p53 in high expression. The devitalization rate: p16 was 66.7%, nm23 was 48.1%, and p53 was 25.9%. In 14 cases, combined mutation of two inhibitory genes were seen. 9 cases were p16 and nm23, among them, 4 cases had transferred to cervical lymph nodes (In all the cases, there were 6 cases who had cervical lymph node transference).

p16 genetic changes are more often detected in oral squamous cell carcinoma than the other genetic changes; In a large portion of cases, there were combined genetic changes instead of single gene mutation.