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[Changes of osteocalcin in serum of young SD rats after functional protrusion]
Objective:
The aim of this study is to investigate temporal and spatial change patterns of osteocalcin in serum of young growing SD rats, in conditions of different functional treatment time spans.
Methods:
Totally, 72 SD rats are randomly divided into 3 groups, including the control group, the 24-hour wearing group (wearing functional appliance 24 hours a day), and the 12-hour wearing group (wearing appliance for 12 hours in daylight). One week after the experiment, rats were sacrificed and osteocalcin level in serum was measured by using radioimmunoassay. Date was processed using macroscopic and microscopic analysis.
Results:
Serum osteocalcin had circadian rhythm and the peak was about 12:00 in a day. After wearing the functional appliance, serum osteocalcin level increased with treatment time, and serum osteocalcin level in the 24-hour wearing group rose more obviously than that in the 12-hour wearing group.
Conclusion:
For the functional appliance, better results can be achieved in the 24-hour wearing group.