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[Relationship between the expression of Bax and apoptosis induced by hyperthermia in BcaCD885 cells]
Abstract
Objective:
This study aimed to investigate the role of Bax gene in apoptosis induced by hyperthermia in BcaCD885 cells.
Methods:
After inducing apoptosis through hyperthermia, the expression of Bax protein and mRNA were examined with FCM antibody analysis and RT-PCR technique respectively.
Results:
Up regulation of Bax protein was noted after hyperthermia. The correlation coefficient r between the apoptotic rate and the expression Bax protein was 0.9240. The expression of Bax mRNA was also raised after hyperthermia.
Conclusion:
Hyperthermia acting as an external signal may up-regulates expression of Bax gene and then induces apoptosis in BcaCD885 cells.