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[Expression of transforming growth factor beta(TGF-beta) subtypes in oral squamous cell carcinoma]
Objective:
The objective of this study was to determine the expression of transforming growth factor beta (TGF-beta) subtypes and their relationship with the mechanisms of squamous cell carcinoma (OSCC) growth.
Methods:
Totally 40 cases of surgical specimens of OSCC resected between 1998 and 2000 and 20 cases of normal human oral mucosa were investigated. Strepto-adridinibiotin complex (SABC) immunohistochemical staining was used to analyze the expression of TGF-beta protein subtypes and their relations with clinical prognosis of OSCC.
Results:
Semi-quantitative analysis revealed that the subtypes 1, 2 and 3 of TGF-beta protein could be found in OSCC cells and normal oral epithelial cells, however the intensity of protein expression was different. Comparing with those in normal oral mucosa epithelial cells, the subtypes 1 and 2 of TGF-beta were over-expressed in OSCC cells. The over-expression of subtypes 1 and 2 of TGF-beta protein were associated with their pathological grades, clinical stages and neck lymph node metastasis (P < 0.05), whilst the subtype 3 protein of TGF-beta was not.
Conclusion:
It will be useful to detect the expression of TGF-beta subtypes in OSCC, as the subtypes 1 and 2 of TGF-beta may play an important role in OSCC growth and metastasis.