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[Regulation of XW630 on TGF-beta 1 and collagen I gene expression in osteoblasts]
1Department of Oral and Maxillofacial Sugery, Sichuan Provincial People's Hospital.
Objective:
The purpose of this part of study was to investigate the mechanism of a new anti-osteoporotic drug, XW630 on promoting osteogenic activity.
Methods:
Total RNA were isolated from osteoblasts of the mice after the reaction with XW630 in 24 and 72 hours, that were hybridized with the extracted and digoxigenin-labeled TGF-beta 1 and the collagen I cDNA probes.
Results:
XW630 significantly promoted TGF-beta 1 and collagen I gene expression in osteoblasts, and this drug was superior than other two kinds of estrogen in promoting osteogenic activity (P < 0.05).
Conclusion:
XW630 promote osteogenic activity of osteoblasts, probably by enhancing the mRNA expression of TGF-beta 1 and collagen I.
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