[Effect of lipopolysaccharide on recombinant human osteogenic protein-1-induced responses in human dental pulp cells]
Related Experiment Video
Updated: Sep 27, 2026
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Objective:
To investigate the effects of lipopolysaccharide (LPS) on recombinant human osteogenic protein-1 (rhOP-1) induced proliferation and differentiation.
Methods:
Measured MTT, alkaline phosphatase (ALPase) activity and osteocalcin content of human dental pulp cells.
Results:
Low-dose LPS (1 microgram/ml) caused co-operative effect with low concentration of rhOP-1 to enhance human dental pulp cell proliferation and ALPase activity, but not to osteocalcin, whereas, high-dose LPS (100 micrograms/ml) significantly inhibited the proliferation, ALPase activity and osteocalcin with or without rhOP-1.
Conclusion:
The role of LPS in caries-induced reparative dentin might be a significant determinant.
