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Related Experiment Videos

Laser capture microdissection as an aid to ultrastructural analysis.

Kenneth Grant1, W Gray Jerome

  • 1Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157-1092, USA.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|January 24, 2003
PubMed
Summary

Laser capture microdissection (LCM) now enables ultrastructural analysis. This method efficiently prepares captured cells for transmission electron microscopy, preserving fine cellular structures.

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Area of Science:

  • Cell biology
  • Microscopy techniques
  • Electron microscopy

Background:

  • Laser capture microdissection (LCM) is a technique for isolating specific cells.
  • LCM was initially developed for genetic analysis and later adapted for proteomic analysis.
  • There was a need to extend LCM applications to ultrastructural studies.

Purpose of the Study:

  • To develop and present a novel method for preparing LCM-captured cells for transmission electron microscopy (TEM).
  • To evaluate the suitability of the new method for high-resolution ultrastructural analysis.

Main Methods:

  • Cells were captured using laser capture microdissection.
  • A new protocol was established for preparing these captured cells for TEM.
  • Standard ultramicrotomy techniques were employed for sectioning.

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Main Results:

  • The developed methodology allows for the preparation of LCM-captured cells for TEM.
  • Cells prepared using this technique exhibit good preservation of fine ultrastructural details.
  • Sections from these cells are amenable to standard ultramicrotomy.

Conclusions:

  • The reported method successfully extends the utility of laser capture microdissection.
  • This technique provides a viable approach for ultrastructural analysis of microdissected cells.
  • The method ensures high-quality sample preparation for detailed electron microscopy studies.