Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antisense oligodeoxynucleotides decrease LGL1 mRNA and protein levels and inhibit branching morphogenesis in fetal

Lami Oyewumi1, Feige Kaplan, Stéphane Gagnon

  • 1Lung Biology Research, Research Institute, The Hospital for Sick Children, 555 University Ave., Toronto, ON, M5G 1X8 Canada.

American Journal of Respiratory Cell and Molecular Biology
|January 24, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The use of bone morphogenetic protein-2 compared with platelet-derived growth factor for the treatment of nonunion : a comparative investigation in a small animal critical-sized defect model.

The bone & joint journal·2025
Same author

The Effect of Cryopreservation on the Bone Healing Capacity of Endothelial Progenitor Cells in a Bone Defect Model.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2025
Same author

Optimization of fs-laser-induced voxels in nonlinear materials via over-correction of spherical aberration.

Optics letters·2024
Same author

Optimal delivery of endothelial progenitor cells in a rat model of critical-size bone defects.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2023
Same author

Smartphone Screen Integrated Optical Breathalyzer.

Sensors (Basel, Switzerland)·2021
Same author

Engineering nanoparticle features to tune Rayleigh scattering in nanoparticles-doped optical fibers.

Scientific reports·2021

The late gestation lung 1 (LGL1) gene product, lgl1, is crucial for fetal rat lung airway branching morphogenesis. Inhibiting LGL1 with antisense oligodeoxynucleotides impaired lung development, confirming its vital role.

Area of Science:

  • Developmental Biology
  • Gene Expression
  • Molecular Biology

Background:

  • The late gestation lung 1 (LGL1) gene is a novel glucocorticoid-inducible gene identified in fetal lung mesenchyme.
  • Previous studies described the cloning of LGL1, but its functional role in lung development was not fully elucidated.

Purpose of the Study:

  • To investigate the role of the LGL1 gene product (lgl1) in fetal rat lung airway branching morphogenesis.
  • To determine the temporal and spatial localization of LGL1 mRNA and lgl1 protein during fetal lung development.
  • To correct the previously published LGL1 sequence.

Main Methods:

  • Reverse transcriptase-polymerase chain reaction (RT-PCR) and in situ hybridization were used to detect LGL1 mRNA.
  • Western analysis and immunohistochemistry were employed to detect lgl1 protein.

Related Experiment Videos

  • Antisense oligodeoxynucleotides were used to inhibit LGL1 function in fetal rat lung explant cultures.

    • Main Results:

    • LGL1 mRNA and lgl1 protein were detected throughout fetal lung development, with increasing expression as gestation progressed.
    • LGL1 expression was localized to mesenchymal and smooth muscle cells.
    • Antisense inhibition of LGL1 dose-dependently inhibited airway branching morphogenesis and reduced lgl1 protein and LGL1 mRNA levels.

    Conclusions:

    • The lgl1 protein plays a significant role in fetal rat lung airway branching morphogenesis.
    • LGL1 expression is temporally and spatially regulated during lung development.
    • Targeting LGL1 offers a potential avenue for understanding and potentially intervening in lung development processes.