[Analysis of 1-(2,6-dichlorophenyl)-2-indolone and related materials by high performance liquid chromatography]

The separation of 1-(2,6-dichlorophenyl)-2-indolone (DCI) and related materials in the synthesis of medical intermediate DCI from 2, 6-dichlorophenol and phenylamine was investigated with high performance liquid chromatography under different chromatographic conditions. The results showed that DCI and related materials were in baseline separation by using CLC-CN(150 mm x 6.0 mm i.d., 7 microns) as column, MeOH-H2O(6:4, V/V) as mobile phase and a flow-rate at 1 mL/min. A rapid, accurate and reproducible HPLC method for determining DCI was developed. Fine and crude DCI samples were detected, results showed that N-(2,6-dichlorodiphenyl)-chloracetyl amide (CBCC) was the main impurity in fine sample, and CBCC and 2,6-dichlorodiphenylamine were the main impurities in the crude one.