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Updated: Aug 24, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
Published on: March 2, 2012
[Study on the separation of trace oxoanions by ion chromatography]
Abstract:
It was the first time that bifunctional (cation exchange/anion exchange) stationary phase was used for the analysis of trace oxoanions by ion chromatography. The influence of sodium phosphate concentration and eluent pH on their separations was studied. An eluent of 7.5 mmol/L Na2HPO4(pH 9.3) was found to be the most suitable for the analysis of SeO3(2-), HAsO4(2-), SeO4(2-), WO4(2-), MoO4(2-), GeO3(2-) and CrO4(2-) simultaneously. An eluent of 0.75 mmol/L Na2HPO4(pH 9.3) was found to be the most suitable for the analysis of IO3-, H2AsO3-, BrO3-, NO2- and NO3- simultaneously. The detection wavelength was 204 nm. Four other anion separation columns (AS4A-SC, AS9-SC, AS11 and PAX 500), which had different structures and properties, were compared to CS5A column. The separation mechanism was also studied.
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