Related Experiment Video
Updated: Aug 17, 2026

Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
Published on: September 7, 2011
[Thermodynamic characteristics of stoichiometric displacement linear parameter log I in reversed-phase liquid
1Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shaanxi Province, Northwest University, Xi'an 710069, China. baiquan@nwu.edu.cn
Abstract:
Based on the physical meaning of each term in the linear parameter log I (a constant correlating the affinity of solute to stationary phase) of stoichiometric displacement model for retention(SDM-R) of solute, the thermodynamic characteristics of log I of solute in reversed-phase liquid chromatography (RPLC) were investigated theoretically. This point was tested by experimental data with two linear relationships: (1) plot of log I vs 1/T (T is absolute temperature) with apolar, polar small solutes and proteins; (2) plot of log I vs log Po/w (partition coefficient of solute between n-octanol and water) with apolar and polar small solutes. From the comparison of the magnitude of partition coefficient between small solute and proteins, a phenomenon that the resolution of small solutes depends on column length but that of proteins almost to be independent of column length is quantitatively explained.
More Related Videos
09:34Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
Published on: September 20, 2016
14:42Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
Related Concept Videos
Distillation: Vapor–Liquid Equilibria
Thin-Layer Chromatography (TLC): Overview
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
Analyte Adsorption and Distribution
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process