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Updated: Sep 21, 2026
08:17
Electrophoretic Separation of Proteins
Published on: June 12, 2008
[Study on the separation of proteins in human serum by high performance capillary zone electrophoresis]
Se Pu = Chinese Journal of Chromatography
|January 25, 2003
Abstract:
A high performance capillary zone electrophoretic method has been developed for the separation of proteins in human serum. Sera were diluted with a buffer (0.05 mol/L boric acid, pH 8.80), and then introduced hydrodynamically into a 37 cm (32 cm to detector) x 50 microns i.d. fused-silica capillary, electrophoresed for 12 min using a boric buffer solution (0.10 mol/L, pH 9.35; containing 4 g/L of polyethylene glycol 8000), and detected at the wavelength of 200 nm. The method is simple, rapid and repeatable.
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