[Recognition and quantitative contrast characteristic components for root of Chinese angelica]

Area of Science:

• Pharmacognosy
• Analytical Chemistry
• Phytochemistry

Background:

• Chinese Angelica root is a vital traditional medicine.
• Accurate quality control is essential for its therapeutic efficacy.
• Existing methods often require reference standards for component identification.

Purpose of the Study:

• To develop a novel method for multi-component quantitative analysis of Chinese Angelica root.
• To establish a reliable quality control strategy independent of reference standards.
• To identify and quantify characteristic liposoluble components in Chinese Angelica root.

Main Methods:

• Reversed-phase High-Performance Liquid Chromatography (HPLC) coupled with a photodiode array (PDA) detector.
• Development of a UV spectra database for component recognition.
• Gradient elution using a 2-propanol/acetonitrile/water mobile phase.
• Analysis at a UV detection wavelength of 202 nm with a flow rate of 1 mL/min on a Hypersil ODS column.

Main Results:

• Successfully recognized 23 characteristic components in two samples of Chinese Angelica root.
• Demonstrated that component concentrations can be similar or different between samples.
• The developed method is convenient, reproducible, and does not require standard components for identification.

Conclusions:

• The established HPLC-UV method provides a robust approach for the quantitative analysis of Chinese Angelica root.
• This technique facilitates quality control by enabling the identification of numerous characteristic components without standards.
• The findings support the reliable assessment of Chinese Angelica root quality for medicinal use.