[The determination sorbitol and carbohydrates in nane fruit by high performance liquid chromatography]

A high performance liquid chromatographic method for the determination of carbohydrates and sorbitol in Nane fruit is described. The chromatography was carried out on a mu-Spherogel carbohydrate column(4.6 mm i.d. x 300 mm) (at 85 degrees C) using water as mobile phase and refractive index detector. The average recovery of carbohydrates were 75.00%-88.37% and correlation coefficients were 0.9709-0.9999. The relative standard deviations for intra-day were 5.10% for sucrose, 3.15% for glucose, 3.03% for fructose and 4.84% for sorbitol respectively (n = 5). The accumulations of soluble carbohydrates(including fructose, glucose, sorbitol and sucrose) in flesh showed their characteristic patterns in different special phase of fruit growth.