[High performance liquid chromatographic determination of domoic acid in shellfish samples]
1Liaoning Entry-Exit Inspection and Quarantine Bureau of China, Dalian 116001, China.
Se Pu = Chinese Journal of Chromatography
|January 25, 2003
Summary
This study presents a validated HPLC method for accurately quantifying domoic acid in shellfish. The developed method ensures reliable detection of this marine toxin, crucial for food safety.
Area of Science:
- Analytical Chemistry
- Food Chemistry
- Marine Toxicology
Context:
- Domoic acid is a potent marine neurotoxin found in shellfish.
- Accurate quantification is essential for public health and regulatory compliance.
- Existing methods may require refinement for routine analysis.
Purpose:
- To develop and validate a High-Performance Liquid Chromatography (HPLC) method for domoic acid determination.
- To establish a reliable analytical procedure for domoic acid in shellfish matrices.
- To achieve a low detection limit for sensitive toxin monitoring.
Summary:
- Shellfish samples were extracted using methanol-water and purified with strong anion exchange solid-phase extraction.
- Domoic acid was quantified using HPLC with a Diode Array Detector (DAD)/UV detector at 242 nm.
- A Zorbax SB-C18 column and an acetonitrile-0.1% aqueous trifluoroacetic acid mobile phase were employed, with external standard calibration.
Impact:
- Provides a validated HPLC-DAD/UV method for sensitive and accurate domoic acid detection.
- Contributes to improved shellfish safety monitoring and risk assessment.
- Offers a robust analytical tool for researchers and regulatory agencies in marine toxin analysis.
