Related Experiment Videos
[Determination of sildenafil in medicines for erectile dysfunction by capillary electrophoresis]
Run-kai Li1, Tao Bo, Hu-wei Liu
1College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Se Pu = Chinese Journal of Chromatography
|January 25, 2003
Abstract:
A method has been developed for the determination of sildenafil in the medicines for erectile dysfunction by capillary electrophoresis. The samples were analyzed with 60 mmol/L NaH2PO4(pH 5.0) running buffer at 35 degrees C capillary temperature and 30 kV voltage. A linear calibration was obtained from 0.07 g/L to 1.05 g/L of sildenafil (r = 0.9985) with the RSD of 4.7% for peak area, and the average recovery was 97.4%. The results were compared with those of HPLC, showing that the method is precise, simple and cost-effective, and can be used as a complementary method to HPLC.