[Quantitative determination of barbaloin in aloe capsule by high performance liquid chromatography]

Barbaloin in aloe capsule was quantitatively determined by high performance liquid chromatography with ODS column, a mixture of CH3OH-H2O (50:50, volume ratio) as mobile phase and UV detection at 298 nm. There was a good linear relationship within the range of 0.02 microgram-1.50 micrograms (r = 0.9999). The recovery was 99.0%-101.9% and the relative standard deviation was 1.37%(n = 6). The detection limit was 0.002 microgram (S/N = 2:1). The total time for separation and determination was within 10 min. The method is rapid, simple, accurate and reproducible. The method is proposed to be used for quality control of aloe capsule.