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[Separation of different phosphatidyl cholines by reversed-phase ion-pair high performance liquid chromatography]
Zhi-hua Wang1, Xue-qing Lu, Xiao-kun Hong
1Shanghai University of Traditional Chinese Medicine and Pharmacology, Shanghai 200032, China.
Se Pu = Chinese Journal of Chromatography
|January 25, 2003
Abstract:
A reversed-phase ion-pair high performance liquid chromatographic (RP-IP-HPLC) method for the separation of different phosphatidyl cholines (PC) was established. The optimized conditions were a PERKIN-ELMER/HS-5 C18 column (150 mm x 4.6 mm i.d., 5 microns), isocratic elution with mobile phase of methanol-acetonitrile-water (70:22:8, volume ratio) containing 15 mmol/L tetramethyl ammonium phosphate (TMAP) at pH 7.0, flow rate of 2.0 mL/min and UV detection at 208 nm. Seven kinds of PC could be separated with this method.