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Related Experiment Videos

Hydration disrupts human stratum corneum ultrastructure.

Ronald R Warner1, Keith J Stone, Ying L Boissy

  • 1Miami Valley Laboratories, Procter & Gamble Co., PO Box 538707, Cincinnati, OH 45253, USA. warner.rr@pg.com

The Journal of Investigative Dermatology
|January 25, 2003
PubMed
Summary

Extended water exposure disrupts the stratum corneum

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Hydration vs. skin permeability to nicotinates in man.

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·2002
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Area of Science:

  • Skin barrier function
  • Dermatology
  • Biophysics

Background:

  • The stratum corneum is the outermost layer of the skin, providing a barrier against environmental factors.
  • Understanding its response to hydration is crucial for skin health and therapeutic applications.

Purpose of the Study:

  • To investigate the effects of prolonged water exposure on the stratum corneum's structure and hydration.
  • To characterize the formation and nature of intercellular water pools (cisternae).

Main Methods:

  • Transmission electron microscopy (TEM)
  • Cryo-scanning electron microscopy (Cryo-SEM)
  • In vivo hydration studies

Main Results:

  • Extended water exposure (4-24 hours) caused significant disruption of intercellular lipid lamellae.
  • Stratum corneum thickness increased 3-4 fold, with less swelling in outer and inner corneocyte layers.
  • Large intercellular water pools (cisternae) formed, disrupting lipid structure and containing significant fluid volume.

Conclusions:

  • Overhydration disrupts the stratum corneum's intercellular space, forming large cisternae.
  • The stratum corneum exhibits more complex and chaotic structures than previously understood.
  • Findings provide insights into desquamation, irritancy, and percutaneous transport.

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