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Endogenous substance P in corneal epithelial cells and keratocytes
Michifumi Watanabe1, Kiyo Nakayasu, Minoru Iwatsu
1Department of Ophthalmology, Asama Municipal Hospital, Nagano, Japan.
Purpose:
To detect endogenous substance P (SP) and neurokinin receptor 1 (NK1R) in cultured human corneal epithelial cells (HE) and cultured human keratocytes (HK).
Methods:
Messenger RNA (mRNA) expression of SP and endogenous SP in HE and HK was investigated by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay. mRNA expression of NK1R in HE and HK was investigated by RT-PCR.
Results:
The mRNA expression of SP and endogenous SP were recognized in HE and HK. Furthermore, the mRNA of NK1R was expressed in HE and HK.
Conclusion:
It was suggested that endogenous SP regulates the biological functions of HE and HK in autocrine or paracrine fashion.