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Hydration pressure and phase transitions of phospholipids. I. Piezotropic approach
H Pfeiffer1, H Binder, G Klose
1Departement Chemie, Katholieke Universiteit Leuven, Celestijnenlaan 200 D, B-3001 Leuven, Belgium. Helge.Pfeiffer@fys.kuleuven.ac.be
Biochimica Et Biophysica Acta
|January 25, 2003
Abstract:
Dehydration reduces the main phase transition pressure of phospholipids. An analysis based on the Gibbs-Duhem equation shows how the shift of the transition pressure is correlated to the hydration pressure. By using Fourier transform infrared (FT-IR) spectroscopy we determined the hydration-dependent phase transition pressure. The application of our new approach gives hydration pressure values which agree with the values obtained with the osmotic stress method.