Related Experiment Video
Updated: Aug 22, 2026

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development
Published on: April 15, 2013
Separation of albendazole sulfoxide enantiomers by chiral supercritical-fluid chromatography
M J del Nozal1, L Toribio, J L Bernal
1Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Prado de la Magdalena s/n, E-47005 Valladolid, Spain. mjdnozal@qu.uva.es
Abstract:
The enantioseparation of albendazole sulfoxide (ABZSO) by chiral supercritical-fluid chromatography (SFC) on two columns, based on the polysaccharide derivatives Chiralpak AD and Chiralcel OD, was studied. The effect of different modifiers, methanol, ethanol, 2-propanol, and acetonitrile, was examined. The results showed that ABZSO can be separated on both columns, using an alcohol-type modifier. Using the Chiralpak AD column, the best results were obtained with 2-propanol and, in the case of the Chiralcel OD, with methanol.
Related Concept Videos
Racemic Mixtures and the Resolution of Enantiomers
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process
Ion-Exchange Chromatography
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...

