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Molecular identification of Paracoccidioides brasiliensis by 5' nuclease assay
Camile P Semighini1, Zoilo P de Camargo, Rosana Puccia
1Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.
Diagnostic Microbiology and Infectious Disease
|January 25, 2003
Abstract:
A PCR assay based on the 5' nuclease assay using a fluorescent probe derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis. The assay could detect at least 10 copies of this DNA sequence, providing efficient accuracy to be useful for diagnosis of paracoccidioidomycosis.