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Updated: Sep 27, 2026

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
Published on: April 5, 2017
Rapid in vivo screening of experimental drugs for tuberculosis using gamma interferon gene-disrupted mice
Anne J M Lenaerts1, Veronica Gruppo, Jason V Brooks
1Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA. lenaerts@colostate.edu
Abstract:
We have developed a rapid new in vivo method for screening experimental drugs for their activity against Mycobacterium tuberculosis by using the gamma interferon gene-disrupted (GKO) C57BL/6 mouse. Due to the rapid growth of the infection, statistical differences indicating positive efficacy of active compounds can be seen after only 8 days of treatment. To validate this model, several fluoroquinolones, including ciprofloxacin, levofloxacin, moxifloxacin, and gatifloxacin, were tested in parallel.

