Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A microbead-based system for identifying and characterizing RNA-protein interactions by flow cytometry.

Alexander S Brodsky1, Pamela A Silver

  • 1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and The Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA. alex_brodsky@dfci.harvard.edu

Molecular & Cellular Proteomics : MCP
|January 25, 2003
PubMed
Summary

This study introduces a high-throughput method using microbeads to identify RNA-protein interactions and pinpoint key nucleotides for binding. This versatile approach accurately detects specific RNA-protein complexes and their binding sites.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering Biosensors to Enhance Monoterpene Indole Alkaloid Production in Yeast.

bioRxiv : the preprint server for biology·2026
Same author

Ligify 2.0: a web server for predicted small molecule biosensors.

Nucleic acids research·2026
Same author

Rational design of selective bispecific EPO-R/CD131 agonists.

Protein engineering, design & selection : PEDS·2025
Same author

groovDB in 2026: a community-editable database of small molecule biosensors.

Nucleic acids research·2025
Same author

Rational design of selective bispecific EPO-R/CD131 agonists.

bioRxiv : the preprint server for biology·2025
Same author

A long-lasting prolactin stimulates galactopoiesis in mice.

iScience·2025

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • Identifying RNA-protein interactions is crucial for understanding gene regulation.
  • Current methods can be low-throughput or lack specificity in complex biological samples.

Purpose of the Study:

  • To develop a high-throughput, versatile method for identifying RNA-protein interactions.
  • To determine specific nucleotides critical for RNA-binding protein recognition.
  • To enable large-scale identification of functionally important RNA sequences.

Main Methods:

  • Coupling oligonucleotides to microbeads for hybridization with RNA-protein complexes.
  • Utilizing fluorescence detection to confirm oligo-RNA-protein complex formation.
  • Analyzing fluorescence patterns to map protein recognition sites on RNA.

Related Experiment Videos

Main Results:

  • The method demonstrates specificity, discriminating between complementary, noncomplementary, and mismatch sequences.
  • Observed fluorescence accurately reflects the affinity and specificity of RNA-protein interactions.
  • Fluorescence patterns successfully footprinted protein recognition sites, identifying key nucleotides.

Conclusions:

  • This microbead-based fluorescence assay is a versatile and high-throughput tool for studying RNA-protein interactions.
  • The system can identify specific RNA sequences crucial for binding RNA-binding proteins.
  • Combined with coded bead systems, this strategy holds potential for genomic-scale RNA-protein interaction mapping.