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Differential effects of exercise training on skeletal muscle SERCA gene expression
Hajime Kubo1, Joseph R Libonati, Zebulon V Kendrick
1Department of Physiology School of Medicine, College of Health, Physical Education, Recreration and Dance, Temple University, Philadelphia, PA 19140, USA.
Introduction:
Exercise training induces significant changes in the performance of skeletal muscle.
Purpose:
To determine whether changes in the gene expression of rat hind-limb muscle sarcoplasmic reticulum Ca2+ -ATPase isoforms (SERCA1a and SERCA2a) in response to either moderate- or high-intensity exercise training underlie the functional remodeling.
Methods:
SERCA1a and SERCA2a isoform mRNA expression was determined in predominantly fast-twitch, gastrocnemius muscle and predominantly slow-twitch, soleus muscle with northern analysis. SERCA mRNA was normalized by the 18S rRNA measured in the same sample.
Results:
Significant increases in the gastrocnemius SERCA2a mRNA expression were observed after both moderate- and high-intensity training. No significant change in SERCA1a expression was found under any conditions.
Conclusion:
These results indicate that both moderate and high intensity exercise increase the relative SERCA2a expression in the gastrocnemius.