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Increased production of leukocyte microparticles with enhanced expression of adhesion molecules from activated

Satoshi Fujimi1, Hiroshi Ogura, Hiroshi Tanaka

  • 1Department of Traumatology, Osaka University Medical School, 2-15 Yamadaoka, Suita City, Osaka 565-0871, Japan. fujimi@hp-emerg.med.osaka-u.ac.jp

The Journal of Trauma
|January 25, 2003
PubMed
Abstract

Insights

Polymorphonuclear leukocyte (PMNL)-derived microparticles (MPs) increase after severe trauma, with enhanced adhesion molecule expression. However, this response may not lead to systemic vascular endothelial damage.

Area of Science:

  • Trauma research
  • Immunology
  • Vascular biology

Background:

  • Polymorphonuclear leukocyte (PMNL)-derived microparticles (MPs) activate vascular endothelium in vitro.
  • Limited understanding of MP production and role in severely injured patients.

Purpose of the Study:

  • Evaluate PMNP-derived MP production in severely injured patients.
  • Clarify the role of these MPs in trauma response.

Main Methods:

  • Serial blood sampling from 30 severely injured patients and 21 healthy volunteers.
  • Flow cytometry to measure MP production, CD11b/CD62L expression, and PMNL oxidative activity.
  • Assessed soluble E-selectin and thrombomodulin as markers of endothelial damage.

Main Results:

  • Increased MP production and PMNL oxidative activity 2-5 days post-trauma.
  • Enhanced CD62L expression on MPs; enhanced CD11b on smaller MPs (<1.0 microm).
  • No significant change in soluble E-selectin or thrombomodulin levels.

Conclusions:

  • Activated PMNLs increase PMNP-derived MP production with elevated adhesion molecules post-severe trauma.
  • This MP response may not directly cause systemic vascular endothelial damage.

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