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Updated: Sep 27, 2026

Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae
Published on: July 19, 2021
Evaluation of Kluyveromyces marxianus as a source of yeast autolysates
Tredwell Lukondeh1, Nicholas J Ashbolt, Peter L Rogers
1School of Civil and Environmental Engineering, The University of New South Wales, UNSW-Sydney 2052, Australia. t.lukondeh@unsw.edu.au
Abstract:
Cells of Kluyveromyces marxianus FII 510700 and Saccharomyces cerevisiae CBS 1907 were autolysed in phosphate buffer, pH 4.5, for a maximum of 10 days to compare chemical changes that occur in the carbohydrate, protein, amino acid and nucleic acid content. Approximately 2.2-3% carbohydrate, 9.5-12% protein, 0.6-1.0% DNA and 6-7% RNA were recovered in the autolysates. The main amino acids were beta-alanine, phenylalanine, cysteine, methionine, glutamic acid and isoleucine. No significant differences in the yeast autolysates of K. marxianus and S. cerevisiae were observed. Consequently, K. marxianus produced from lactose-based media has potential as a source of yeast autolysates used in the food industry.
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