[Quantitative analysis of sparfloxacin injection by high performance liquid chromatography]

Analytical method of the quantitative determination of sparfloxacin injection by HPLC is described. The analytical conditions were as follows. A Waters Symmetry C18(5 microns, 150 mm x 3.9 mm i.d.) column was used as the analytical column. The detection wavelength was UV-298.8 nm. The column temperature was 30 degrees C. The mobile phase was 0.2% KH2PO4 buffer (pH 3.2)-CH3CN-CH3OH (80:15:5, volume ratio) and the flow-rate was 1.0 mL/min. The injection volume was 10 microL. The linear range (the peak area vs. the mass concentration of sparfloxacin) was from 39.94 mg/L to 199.68 mg/L, and the correlation coefficient was 0.9999. The average recovery of sparfloxacin was 100.1% (n = 5), and its RSD was 0.72%. The RSDs of continuous injections, within day injections per 2 hours and between day injections in three days were 0.19%, 0.14% and 0.13% respectively. The above analytical results show that this method has good precision and stability. It is a rapid, sensitive and accurate method for the analysis of sparfloxacin.