Related Experiment Video
Updated: Aug 18, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
[Analysis of soybean lecithin by supercritical fluid chromatography]
1State Key Laboratory of Heavy Oil Research, Petroleum University, Beijing 102200, China.
Abstract:
Separation of six phospholipids, phosphatidyl choline (PC), phosphatidyl-ethanolamine (PE), phosphatidyl-serine(PS), phosphatidyl-inositol(PI), phosphotidic acid(PA), lyso-phosphatidyl-choline(lyso-PC), in soybean lecithin with supercritical fluid chromatography was achieved within 15 min. C18 column was used and carbon dioxide modified by ethanol containing 0.05% (V/V) triethylamine was chosen as the mobile phase. Effects of the composition of mobile phase, temperature and pressure were studied. The quantitative analysis of PC has been achieved with external standard method. The calibration curve for PC was linear in the range between 0.020 g/L-0.075 g/L and the detection limit was 0.2 microgram. This method has been applied to the analysis of PC in soybean lecithin.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Instrumentation
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

