[Study on the developmental toxicity of Bisphenol A by using micromass culture in vitro]

The micromass culture of Wistar rat embryo limb bud cells was used to investigate the characteristic of developmental toxicity of Bisphenol A (BPA) and its mechanism in vitro. The results showed that BPA inhibited both proliferation and differentiation of rat limb bud cells in vitro. The high level of BPA appeared to be cytotoxic to Wistar rat embryo limb bud cells in culture and inhibited the clone formation with dose-response relationship. The concentration of BPA for IP50 and ID50 were 38.74 mg/L and 27.93 mg/L respectively. According to Renaults' teratogenic criteria, BPA belonged to a positive teratogen. And the data suggested that the specific inhibition of cell proliferation and differentiation might be one of the mechanisms of high level BPA.