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Anti-inflammatory effects of aqueous extract from Dichroa febrifuga root in rat liver
Byung-Tae Choi1, Jun-Hyuk Lee, Woo-Shin Ko
1Department of Oriental Medicine, College of Oriental Medicine, Research Institute of Oriental Medicine, Dong-Eui University, Busan 614-052, Korea. choibt@dongeui.ac.kr
Aim:
To study the anti-inflammatory effects of aqueous extract from Dichroa febrifuga root (AEDF) for suppression in the process of lipopolysaccharide (LPS)-induced sepsis in the rat liver.
Methods:
The inhibitory effect of AEDF on the alteration of inflammatory proteins was investigated by Western blot and immunohistochemical analysis.
Results:
Western blot analysis showed that the level of nuclear factor (NF)-kappaBp65 was markedly up-regulated and (I)-kappaBalpha was down-regulated by LPS (8 mg/kg) challenge. However, AEDF 100 mg/kg inhibited induction of NF-kappaBp65 and degradation of I-kappaBalpha in the liver of LPS-challenged rats. Immunohistochemical analysis showed that while the expression of the NF-kappaBp65, tumor necrosis factor (TNF)-alpha and inducible nitric oxide synthase (iNOS) tended to increase, that of I-kappaBalpha was decreased in the hepatocytes of rats challenged with LPS. A slight decline of NF-kappaBp65, TNF-alpha and iNOS, but an increase of I-kappaBalpha were observed in the hepatocytes of the rats pretreated with AEDF.
Conclusion:
AEDF may act as a therapeutic agent for inflammatory disease through a regulation of inflammation-related proteins.