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Rat erythrocytes express the anionic amino acid transport protein EAAC1
Donald Novak1, Mark Beveridge, Jill Verlander-Reed
1Division of Pediatric Gastroenterology and Hepatology, University of Florida College of Medicine, Gainesville, FL 32610, USA. novakda@peds.ufl.edu
Blood Cells, Molecules & Diseases
|January 28, 2003
Abstract:
EAAC1 is a widely expressed protein which transports anionic amino acids in a Na(+)-dependent fashion. Rat erythrocytes have generally been thought to be impermeant to anionic amino acids. Utilizing immunoelectron microscopy, we have demonstrated the presence of EAAC1-immunoreactive protein within rat erythrocytes. Immunoblotting revealed the presence of an approximately 60-kDa protein, consistent with EAAC1, in erythrocyte membranes. Specificity was confirmed by peptide competition. In conclusion, EAAC1 is expressed in rat erythrocytes.