Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

741
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
741
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.8K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.8K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multinodular Hydropic Leiomyoma in a 41-Year-Old Patient: A Case Report.

Journal of clinical medicine·2025
Same author

From gaps to compliance: a 12-year retrospective cohort study of trends in mismatch repair protein testing and Lynch syndrome identification in colorectal cancer in Central Switzerland.

Swiss medical weekly·2025
Same author

Validation and clinical performance of a single test, DNA based endometrial cancer molecular classifier.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society·2025
Same author

cfDNA UniFlow: a unified preprocessing pipeline for cell-free DNA data from liquid biopsies.

GigaScience·2024
Same author

LBFextract: Unveiling transcription factor dynamics from liquid biopsy data.

Computational and structural biotechnology journal·2024
Same author

Validation and clinical performance of a single test, DNA based endometrial cancer molecular classifier.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society·2024

Related Experiment Video

Updated: Jan 10, 2026

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
10:25

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Published on: September 27, 2024

1.0K

Multicolor deconvolution microscopy of thick biological specimens.

Christine Maierhofer1, Rainer Gangnus, Joachim Diebold

  • 1Institut für Humangenetik, Technische Universität München, München, Germany.

The American Journal of Pathology
|January 28, 2003
PubMed
Summary

This study introduces a novel multicolor deconvolution technique for analyzing DNA at the single-cell level within tissues. This method enables high-resolution imaging, advancing cancer cell biology and chromosomal analysis.

More Related Videos

Application of MultiColor FlpOut Technique to Study High Resolution Single Cell Morphologies and Cell Interactions of Glia in Drosophila
08:30

Application of MultiColor FlpOut Technique to Study High Resolution Single Cell Morphologies and Cell Interactions of Glia in Drosophila

Published on: October 20, 2017

8.6K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.3K

Related Experiment Videos

Last Updated: Jan 10, 2026

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
10:25

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Published on: September 27, 2024

1.0K
Application of MultiColor FlpOut Technique to Study High Resolution Single Cell Morphologies and Cell Interactions of Glia in Drosophila
08:30

Application of MultiColor FlpOut Technique to Study High Resolution Single Cell Morphologies and Cell Interactions of Glia in Drosophila

Published on: October 20, 2017

8.6K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.3K

Area of Science:

  • Cellular Biology
  • Genomics
  • Microscopy

Background:

  • Understanding cellular-level disease mechanisms is limited by the inability to analyze DNA within natural tissue contexts.
  • Single-cell DNA analysis is crucial for cancer research, evolution studies, and understanding rare cellular events.

Purpose of the Study:

  • To present a novel fluorescence in situ hybridization-based multicolor deconvolution technique.
  • To enable high-resolution, multi-channel DNA analysis at the single-cell level within 3D tissue samples.

Main Methods:

  • Utilized a fluorescence in situ hybridization (FISH) based multicolor deconvolution technique.
  • Employed up to seven different color channels for probe detection.
  • Integrated a DNA counterstain for simultaneous nuclear segmentation and volume labeling.

Main Results:

  • Achieved simultaneous high-resolution localization of multiple point-like sources in biological specimens up to 30 micrometers thick.
  • Enabled detailed analysis of DNA on a cell-to-cell basis within the natural tissue context.
  • Developed a low-cost alternative to confocal microscopy using standard fluorescence microscopy instrumentation.

Conclusions:

  • The presented technique overcomes limitations in single-cell DNA analysis within tissues.
  • This method provides valuable insights into cancer cell biology, evolution, and chromosomal mosaicism.
  • Offers a cost-effective, high-resolution imaging solution for cellular and genomic research.