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04:18
A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction
Published on: May 17, 2024
[Cloning and sequencing of the Chinese bovine prion protein (PrPC) gene]
Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|January 29, 2003
Abstract:
The total DNA was isolated from lymphocyte of peripheral blood of Chinese cattle. The PrPC gene was amplified by polymerase chain reaction, using two primers. It was then cloned into pGEM-T Easy Vector. The result of DNA sequencing was indicated that the 795 bp amplified fragment contains the entire PrPC coding sequence, which has no intron and is same as the published gene sequence.
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