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[Continuous ethanol fermentation using tapioca starch by immobilized yeast cell without carrier].

Y Ma1, X Zhou, P Wu

  • 1East China University of Science and Technology, Shanghai 200237.

Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|January 29, 2003
PubMed
Summary

This study demonstrates efficient continuous ethanol fermentation using self-flocculating yeast. The two-stage system achieved high ethanol yields and productivity from tapioca syrup, showcasing its stability over a month.

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Area of Science:

  • Biotechnology
  • Fermentation Science
  • Yeast Physiology

Background:

  • Continuous ethanol fermentation is gaining interest for industrial applications.
  • Immobilized yeast systems, particularly self-flocculating strains, offer potential advantages.
  • Schizosaccharomyces pombe is a yeast species known for its flocculation properties.

Purpose of the Study:

  • To investigate continuous ethanol fermentation using a naturally flocculent strain of Schizosaccharomyces pombe.
  • To establish and evaluate a two-stage continuous fermentation system for ethanol production.
  • To assess the system's performance in terms of ethanol concentration, residual glucose, productivity, and substrate conversion.

Main Methods:

  • Utilized a naturally flocculent strain of Schizosaccharomyces pombe.

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  • Implemented a two-stage continuous fermentation system with suspended-bioreactors (1.5 L effective volume).
  • Fed the system with tapioca syrup at a glucose concentration of 150 g/L.

    • Main Results:

    • Achieved high biomass levels, enabling efficient ethanol conversion (average 72.7 g/L).
    • Maintained low residual glucose levels (average 3.74 g/L).
    • Reached high productivity (9.7 g/L.h) with an average 90% total substrate conversion.

    Conclusions:

    • The established two-stage continuous fermentation system is effective for ethanol production.
    • High biomass levels achieved with self-flocculating yeast contribute to efficient substrate conversion.
    • The system demonstrated stable performance over a one-month research period.