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[Effects of GDNF on primary culture of spinal cord neurons]
1Department of Neurobiology, Second Military Medical University, Shanghai 200433.
Shi Yan Sheng Wu Xue Bao
|January 29, 2003
Abstract:
The effect of GDNF on long-term cultured spinal cord neurons was studied. GDNF could promote spinal cord neurons survival after 7 d or 14 d culture by MTT assay. The effect of GDNF on growth cones, neuron soma magnitude, neurite length and spines formulation of spinal cord neurons in cell culture was observed by phase microscopy, Nissl stain and NSE immunocytochemistry stain. The results indicated that GDNF had significant trophic effects on long-term cultured spinal cord neurons.
