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[Studies on monoclonal antibodies of Magnaporthe grisea and its interfering effect on appressorium formation]

J Wu1, F Liu, D Li

  • 1Institute of Biotechnology, Zhejiang University, Hangzhou 310029.

Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|January 29, 2003
PubMed

Selecting by indirect ELISA eleven hybridoma cell lines, secreting monoclonal antibodies (McAbs) against Magnaporthe grisea were produced by fusing mouse myeloma cells(SP2/0) with spleen cells from BALB/c mice immunized with the mixture of conidia, germ tubes and appressoria of M. grisea using 50% PEG. The result of IFTC test showed that four of McAbs, named as 2B4, 4A1, 1D1, and 2H4 specifically bound to the cell wall surface of the fungus; Western blotting revealed that 2B4, 4A1, 1D1 were recognized different protein antigens from the surface of conidia and germ tubes; These four McAbs could effectively interfere with the appressorium formation both on cellophane membrane and the surface of onion epidermis and inhibit the disease leaf lesions development in vitro test.

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