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[High concentration ethanol continuous fermentation using yeast flocs].

C Liu1, F Bai, M Shao

  • 1Institute of Biochemical Engineering, Dalian University of Technology, Dalian 116012, China.

Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|January 29, 2003
PubMed
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Continuous ethanol fermentation using yeast flocs achieved high ethanol concentrations (96.6 g/L) in a series of bioreactors. This process efficiently utilized sugars, leaving minimal residual amounts, demonstrating a robust fermentation strategy.

Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Microbiology

Background:

  • Continuous fermentation processes are crucial for industrial ethanol production.
  • Yeast flocs offer advantages in cell retention and volumetric productivity.
  • Air-lift suspended-bed bioreactors provide efficient mass transfer and mixing.

Purpose of the Study:

  • To investigate the efficacy of continuous ethanol fermentation using yeast flocs.
  • To optimize fermentation conditions for high ethanol yield and sugar utilization.
  • To evaluate the performance of air-lift suspended-bed bioreactors in series.

Main Methods:

  • Utilized yeast flocs in four serially operated air-lift suspended-bed bioreactors.
  • Maintained continuous fermentation under CO2 atmosphere.

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  • Implemented complete recycle of ethanol distilled effluent broth.
  • Operated at a dilution rate of 0.2/h.

    • Main Results:

    • Achieved an average ethanol concentration of 96.6 g/L in the fermentation broth.
    • Demonstrated high sugar conversion with residual total sugar at 4.1 g/L.
    • Reported residual reducing sugar concentration of 1.2 g/L, indicating efficient fermentation.

    Conclusions:

    • Continuous ethanol fermentation with yeast flocs in series is highly effective.
    • The system achieved high ethanol titers and efficient sugar consumption.
    • Air-lift suspended-bed bioreactors are suitable for large-scale, continuous bioethanol production.