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[Advances in affinity chromatography].

J Zhang1, Z Jiang, H Chen

  • 1Institute of Radiation Medicine, Academy of Military Medical Science, Beijing, 100850.

Se Pu = Chinese Journal of Chromatography
|January 29, 2003
PubMed
Summary
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This review covers recent advances in affinity chromatography, including new ligand discovery techniques and improved systems. It highlights key applications of this powerful separation method.

Area of Science:

  • Biochemistry
  • Separation Science
  • Analytical Chemistry

Context:

  • Affinity chromatography is a crucial technique for biomolecule purification.
  • Advances in ligand discovery and system optimization are essential for its efficacy.
  • This review focuses on the latest developments in the field.

Purpose:

  • To provide a comprehensive overview of recent progress in affinity chromatography.
  • To discuss novel techniques for ligand discovery, including combinatorial chemistry and molecular imprinting.
  • To highlight improvements in chromatographic supports and systems, and explore diverse applications.

Summary:

  • Recent advances in affinity chromatography are reviewed, focusing on mechanisms and ligand discovery.
  • New techniques like combinatorial chemistry, molecular imprinting, de novo design, and fusion expression for ligand discovery are discussed.

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  • Improvements in chromatographic supports, systems, and the broad applications of affinity chromatography are also covered.

    • Impact:

    • This review offers insights into cutting-edge affinity chromatography methods.
    • It serves as a valuable resource for researchers seeking to optimize purification strategies.
    • Understanding these advances can lead to more efficient and targeted biomolecule isolation.