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Updated: Sep 27, 2026

High-Throughput Screening of L-Lactic Acid-Hyperproducing Bacillus coagulans Mutants Using an Automated Droplet Microfluidic Platform
Published on: July 28, 2026
[The construction and application of promoter-signal peptide probe vector of lactic acid bacteria]
1State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Abstract:
A promoter-signal peptide probe vector of lactic acid bacteria, designated pZLB based on pMG36e has been constructed. The vector contained a reporterd gene encoding for beta-lactamase, which lacked a promoter and a functional signal sequence. Sau3A restriction fragments (80-400 bp) from L. lactis ATCC 7962 total DNA were cloned into BamHI site of pZLB. A lot of different size framents were selected. These fragments promoted transcription and secretion of beta-lactamase in L. lactis. Sequence analysis revealed that inserted fragment in pZLBe7 contained typical promoter, SD sequence and atypical signal sequence.

