[Specific amplification of the coding sequences of BBTV III, IV, I and their application in BBTV detection]

Banana bunchy top virus disease (BBTD) is a disastrous disease in bananas, and it is spreading in the world (including China) by the banana bunchy top virus(BBTV). At present, virus-free plantlets are used to prevent BBTD in banana production, therefore, it is very important to establish a method to detect BBTV quickly, sensitively and specifically. ELISA is now popularly used to detect BBTV. The sensitivity of this method is not high enough, and needs specific antiserum, otherwise, pseudo-positive results often occur. According to DNA coding sequences of component III, IV and I of BBTV isolates from Zhangzhou, China, three pairs of primers are designed to establish a PCR method to specifically amplify parts of coding sequences of the BBTV coat protein, movement protein and replicase-association. This method is also applicable to detect BBTV of bananas or cultured banana seedlings in other regions.