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Updated: Sep 27, 2026

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Preparation of Quality Inositol Pyrophosphates
Published on: September 3, 2011
[Purification and properties of intercellular inorganic pyrophosphatase from Saccharomyces cerevisiae]
Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|January 29, 2003
Abstract:
An inorganic pyrophosphatase (EC3.6.1.1) from Saccharomyces cerevisiae was purified to PAGE homogeneity by sonication disruption, (NH4)2SO4 fractionation and DEAE-cellulose column chromatography. The optimum pH and temperature of the enzyme were 7.4-7.8 and 60 degrees C, respectively. The Km was 19.3 mmol/L. The enzyme required Mg2+ as a cofactor for hydrolysis of pyrophosphate and was inhibited by Ca2+, Hg2+, Pb2+, Mn2+.

