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Updated: Sep 27, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
09:42

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

[Establishment of hybridoma secreting anti-mycobacteria monoclonal antibody by using electrofusion technique]

M Guo1, Z Pan, H Wang

  • 1Department of Medical Imaging, Tianjin Medical University, Tianjin 300203.

Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|January 29, 2003
PubMed

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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...