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[Characterization of the Streptomyces plasmid pSGL1]
1Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100050.
Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|January 29, 2003
Summary
The plasmid pSGL1 was characterized from Streptomyces globisporus, revealing a minimal replication region. Its derivatives show potential as versatile cloning vectors for Streptomyces.
Area of Science:
- Molecular biology
- Microbial genetics
Context:
- Characterization of plasmid pSGL1 (7.4 kb) isolated from Streptomyces globisporus C-1027, a producer of the antitumor antibiotic C-1027.
- Successful introduction of pSGL1 into Streptomyces lividans via transformation.
Purpose:
- To characterize the plasmid pSGL1 and identify its minimal replication region.
- To evaluate the potential of pSGL1 derivatives as cloning vectors.
Summary:
- The minimal replication region of pSGL1 was localized to a 2.0 kb Sau 3AI fragment.
- pSGL1 is compatible with pIJ101 and exhibits a copy number of 70-250.
- Derivatives like pSGLN and pSGLS3 possess selectable antibiotic resistance markers and unique restriction sites, making them suitable for cloning.
Impact:
- pSGL1 derivatives can be utilized as efficient cloning vectors in Streptomyces.
- These plasmids offer potential for the design and construction of secretory expression vectors in Streptomyces species.