[Analysis of higher basidiomycetous mitochondrial DNA with four-GC-cutter restriction enzymes]

The mitochondrial DNA band patterns emerged from basidiomycetous genomic DNA digested with four-GC-cutter restriction enzymes, HaeIII, CfoI and MspI, were reported in this paper. It was demonstrated that mtDNAs of higher basidiomycetes had abundant diversity after comparison of their band patterns, summated sizes, the number of fragments and homology among 12 strains of Agaricales, Aphyllophorales. The results also showed that the technique, displaying fungal mtDNA bands directly from their total DNA, would be more efficient and sensitive for analyses of fungal mtDNA RFLP if several four-GC-cutter restriction enzymes, instead of one, were used.