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[Isolation of a lipase-producing Pseudomonas strain and optimization of its fermentation conditions]
Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|January 29, 2003
Abstract:
A lipase-producing bacterium strain was isolated from soil and was identified as Pseudomonas sp.. Its lipase yield was improved 2.25-fold by combined treatment of UV irradiation and NTG. The lipase fermentation condition for the mutant strain was optimized with Plackett-Burman design and Response Surface Analysis (RSA), and the formula of the optimum medium suitable for industrial scale fermentation was thereby established. A maximum yield of 87.5 U/ml was obtained.