Jove
Visualize
Contact Us

Related Experiment Videos

Multiple calmodulin genes in fish.

Felix Friedberg1, Allen R Rhoads

  • 1Howard University College of Medicine, 520 W St., N.W., Washington, DC 20059, USA.

Molecular Biology Reports
|January 29, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single and multiple CH (calponin homology) domain containing multidomain proteins in Arabidopsis and Saccharomyces: an inventory.

Molecular biology reports·2010
Same author

Single and multiple CH (calponin homology) domain containing multidomain proteins in Dictyostelium discoideum: an inventory.

Molecular biology reports·2009
Same author

Duplex (or quadruplet) CH domain containing human multidomain proteins: an inventory.

Molecular biology reports·2009
Same author

Singlet CH domain containing human multidomain proteins: an inventory.

Molecular biology reports·2009
Same author

Alternative splicing for members of human mosaic domain superfamilies. I. The CH and LIM domains containing group of proteins.

Molecular biology reports·2008
Same author

Centrin isoforms in mammals. Relation to calmodulin.

Molecular biology reports·2006
Same journal

Neuroprotective potential of resveratrol in Parkinson, Huntington, amyotrophic lateral sclerosis, and multiple sclerosis: a comprehensive review.

Molecular biology reports·2026
Same journal

IL-22 modulates STAT3/β-catenin/let-7a signaling and hepatic granulomas in Schistosoma mansoni infection: versus praziquantel and combination treatment.

Molecular biology reports·2026
Same journal

Stem cell-based regenerative therapies for non-ischemic cardiomyopathy: mechanistic insights, clinical evidence, and future directions.

Molecular biology reports·2026
Same journal

A novel DREB transcription factor enhances salt tolerance in peanut (Arachis hypogaea L.) through upregulation of AhP5CR and AhBADH, leading to increased osmolyte accumulation.

Molecular biology reports·2026
Same journal

Retraction Note: 2-Hydroxylethyl methacrylate (HEMA), a tooth restoration component, exerts its genotoxic effects in human gingival fibroblasts trough methacrylic acid, an immediate product of its degradation.

Molecular biology reports·2026
Same journal

Development of a CRISPR/dCas9-based membrane-assisted colorimetric assay for detection of high-risk HPV16 and HPV18: a proof-of-concept study.

Molecular biology reports·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Mammals and fish share a multi-gene system for producing identical calmodulin (CaM) proteins. This study identified three distinct CaM genes in zebrafish, confirming this system extends beyond mammals to other vertebrates.

Area of Science:

  • Molecular Biology
  • Evolutionary Biology
  • Genetics

Background:

  • Mammals possess three nonallelic genes encoding identical calmodulin (CaM) proteins, differing primarily in their untranslated regions (UTRs).
  • The specific UTRs are highly conserved within mammalian species, suggesting functional importance.

Purpose of the Study:

  • To investigate if the mammalian multi-gene, single-protein system for calmodulin extends to other vertebrate groups, specifically fish.
  • To identify and characterize calmodulin genes in fish species and compare their UTRs with those in mammals.

Main Methods:

  • Utilized GenBank and EST databases for sequence retrieval and performed direct sequencing.
  • Designed and employed PCR primers targeting coding and 3'-UTR regions to confirm gene presence in zebrafish cDNA libraries.

Related Experiment Videos

  • Conducted phylogenetic analysis to assess evolutionary relationships among fish calmodulin genes and with mammalian counterparts.

    • Main Results:

    • Identified three distinct calmodulin genes (alpha, beta, gamma) in zebrafish (Danio rerio), which encode identical CaM proteins but differ in their 3'-UTRs.
    • Confirmed the presence of homologous calmodulin genes in other fish species, showing close evolutionary relationships.
    • Observed that fish 3'-UTRs are less conserved than mammalian UTRs, with only a few short conserved elements shared between fish and mammalian calmodulin genes.

    Conclusions:

    • The multi-gene, single-protein system for calmodulin production is conserved in fish, similar to mammals.
    • Evolutionary divergence has led to reduced conservation in fish 3'-UTRs compared to mammals.
    • Identified specific short sequence similarities in 3'-UTRs between fish and mammalian calmodulin genes, hinting at potential shared regulatory elements.