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Molecularly imprinted polymer as storage medium for an analyte.
1Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Poojapura Trivandrum-695012, India.
Bioseparation
|January 29, 2003
Summary
Molecularly imprinted polymers (MIPs) can selectively bind theophylline. These MIPs demonstrate quantitative theophylline desorption after storage, indicating their potential as analyte storage matrices.
Area of Science:
- Polymer Chemistry
- Analytical Chemistry
- Materials Science
Background:
- Molecularly imprinted polymers (MIPs) are synthetic receptors with tailored binding sites.
- Noncovalent imprinting offers a versatile route for creating selective polymer matrices.
- Theophylline is a pharmaceutical compound requiring accurate quantification.
Purpose of the Study:
- To develop a selective molecularly imprinted polymer (MIP) for theophylline.
- To evaluate the potential of MIPs as a storage matrix for theophylline.
- To assess the quantitative desorption and analysis of stored theophylline.
Main Methods:
- Preparation of a noncovalent molecularly imprinted polymer (MIP) using theophylline as a template.
- Equilibration of the MIP with theophylline.
- Storage of the theophylline-loaded MIP for varying time periods.
- Quantitative desorption and analysis of the stored theophylline.
Main Results:
- The developed MIP exhibited selective binding of theophylline.
- Quantitative desorption of theophylline was achieved after storage.
- The amount of desorbed theophylline was consistent over time, confirming stability.
- The MIP proved effective as a storage matrix for the analyte.
Conclusions:
- Noncovalent MIPs can be effectively utilized for selective theophylline binding.
- MIPs serve as a viable storage matrix for analytes like theophylline.
- The stored analyte can be conveniently desorbed and analyzed, enabling reliable quantification.